Fig. 6
From: The cytokines interleukin-6 and interferon-α induce distinct microglia phenotypes
Microglia in the brain of GFAP-IL6 versus GFAP-IFN mice have unique surface marker expression profiles. a–e From 1-, 3- and 6-month-old MacGreen-WT, -GFAP-IL6 and -GFAP-IFN mice, microglia were gated (live eGFP+ CD45low CD11b+ Ly6C– TMEM119+ cells) and the median fluorescence intensity (MFI) was quantified for a TMEM119, b CD16/32, c CD64, d CD11b and e SCA-1 levels. f–h In a separate experiment, microglia were gated (live eGFP+ CD11b+ 4D4+ cells or live eGFP+ CD11b+ FCRLS+ cells) and the MFI was quantified for f 4D4, g FCRLS and h MHC-I levels. i-o Percentages of microglia positive for i CD64, j CD11c, k SCA-1, l MHC-I, m MHC-II, n CD80 and o CD86. n = 3–5 mice/group. Graphs show individual values per mouse and mean ± SEM. *, p < 0.05 compared with WT; ^, p < 0.05 compared with GFAP-IL6; #, p < 0.05 compared with 1-month-old of same genotype; x, p < 0.05 compared with 3-month-old of same genotype using two-way ANOVA with Tukey’s post-test. † N.B. The results for 6-month-old microglia are not shown due to high autofluorescence interference and are therefore not reliable