Fig. 4

PDGFD is mainly secreted by macrophages/microglia and fibroblasts after SCI. A–D Immunofluorescence staining of PDGFD (red), CD68 (green) and PDGFRβ (white) in sagittal sections at 14 dpi. E–H Higher magnification images of the boxed region in D. Arrows indicate CD68⁺PDGFD⁺ cells. Arrowheads indicate PDGFRβ⁺PDGFD⁺ cells. I–L Immunofluorescence staining of PDGFD (red), CD31 (green) and nuclei (blue) in sagittal sections at 14 dpi. J–L represents boxed region in I. M–P Immunofluorescence staining of PDGFD (red), GFAP (green) and nuclei (blue) in sagittal sections at 14 dpi. N–P represents boxed region in M. Q Quantification of the proportion of CD68⁺PDGFD⁺ cells, PDGFRβ⁺PDGFD⁺ cells or CD31⁺PDGFD⁺ cells in PDGFD⁺ cells at 14 dpi. Asterisks indicate the injured core. Scale bars: 200 μm in D, I and M and 10 μm in H, L and P. n = 5 animals per group