Fig. 9

Intrathecal injection of SU16f promotes axon regeneration after SCI. A–H Immunofluorescence staining of NF (red) and GFAP (green) in sagittal sections of the Control and SU16f groups at 28 dpi. The region of interest (ROI) represents the boxed region on the left. I–N Immunofluorescence staining of 5-HT (red) and GFAP (green) in sagittal sections of the Control and SU16f groups at 28 dpi. O–Q Higher magnification images of the boxed region in J and M. R Quantification of the density of NF⁺ axons in the GFAP⁻ area at 28 dpi. S Quantification of the percentage of 5-HT⁺ area in the area of the spinal cord segment spanning the injured core at 28 dpi. Asterisk indicates the injured core. Scale bars: 200 μm in G and N, 20 μm in H and 50 μm in Q. *P < 0.05 and ****P < 0.0001 by Student’s t test, n = 5 animals per group