Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: miR-203, fine-tunning neuroinflammation by juggling different components of NF‐κB signaling

Fig. 3

miRNA-203 activated microglia and promoted inflammatory gene expression. A Representative confocal Z-stack images for Iba1 (red) in CA3 and DG of mouse hippocampus overexpressing miR203 or nonsense scrambles. The scale bar was 50 μm. B The skeleton analysis of microglia morphology. Summed microglia endpoints were reduced in the mouse hippocampus with ectopic expression of miR-203, compared with controls expressing nonsense scrambles. Results of three consecutive sections consisting of four views of bilateral CA3 and DG of three independent biological replicates were presented, with the height of columns representing the mean and the error bars showing the S.D. C Quantification of microglia cell numbers. Results of three consecutive sections consisting of four views of bilateral CA3 and DG of three independent biological replicates were presented, with the height of columns representing the mean and the error bars showing the S.D. D, HJ Western blots showed increased expression of Iba1, IL-6 and TNF-α in mouse hippocampus with ectopic expression of miR-203, compared with that of controls expressing nonsense scrambles and untransfected blank controls. Luminescence-based relative quantification of protein (Iba1:β-actin; IL-6:β-actin; TNF-α:β-actin) are expressed as mean ± SD of three biological replicates (HJ) (one-way ANOVA, Turkey’s post hoc). E Area percentages of Iba1 (red) in the DG and CA3 were significantly increased in mouse hippocampus with ectopic expression of miR-203, compared with that of controls expressing nonsense scrambles. Results of three consecutive sections consisting of four views of bilateral CA3 and DG of three independent biological replicates were presented, with the height of columns representing the mean and the error bars showing the S.D. F, L Immunofluorescence showed increased IL-6 (red) in mouse hippocampus with ectopic expression of miR-203 (green), compared with controls expressing nonsense scrambles. Quantification of IL-6 positive areas of three consecutive sections consisting of four views of bilateral CA3 and DG of three independent biological replicates were presented, with the height of columns representing the mean and the error bars showing the S.D. G, M Immunofluorescence showed increased IL-1β (red) in mouse hippocampus with ectopic expression of miR-203 (green), compared with controls expressing nonsense scrambles. Quantification of IL-1β positive areas of three consecutive sections consisting of four views of bilateral CA3 and DG of three independent biological replicates were presented, with the height of columns representing the mean and the error bars showing the S.D. Nuclei were stained with Hoechst 33342 (blue) and the scale bar was 150 μm. Asterisks indicate statistical significance between samples (*P < 0.05; **P < 0.01; ***P < 0.001)

Back to article page