Fig. 3

NLRX1-mediated incomplete mitophagy in morphine-treated BV2 cells. A Confocal microscopy analysis of MitoTracker Green Tm and LysoTracker Red DND-99 in BV2 cells of NC-siRNA or NLRX1-siRNA group at the absent or present of morphine. Bar = 5 μm. Quantitative graphs of mitochondrial form factors (B), average sizes (C) and lysosomal diameters (D) as measured by Image J (n > 25 cells). E–F Representative images of confocal microscopy analysis of LAMP1 and LC3B and quantitative analysis of overlap coefficients, lysosomal phagocytosis ratio of LC3B and LC3 + phagosomes (F, n = 24–36 cells). Bar = 2 μm. Data represent the mean ± SEM. Student's t-test or Mann–Whitney U test were used to measure significance between two groups. (* p < 0.05, ** p < 0.01, *** p < 0.001 and ns p > 0.05)