Fig. 2

LCN2 mainly expressed on neurons. a Representative immunofluorescence images of LCN2 (green), GFAP (red), NeuN (red) and IbaI (red) staining in sections of the hippocampus after surgery. Long scale bar, 600 µm; short scale bar, 25 µm. b Percentage quantification of LCN2 positive cells (n = 3 mice/group). c Cartoon depicting MACS sorting procedures for isolating CD11b⁺ and CD11b ⁻ cells. d Relative expression of cx3cr1 in CD11b ⁺ and CD11b ⁻ cells after sorting from all samples. d Relative expression level of Lcn2 mRNA in CD11b ⁺ and CD11b ⁻ cells from hippocampus 6 h after surgery. e Relative expression level of IL-6 mRNA in CD11b ⁺ cells. Data are represented as mean ± SEM. **** P < 0.0001