Fig. 3
From: Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia
Elevation of LCN2 is independent of the presence of microglia. a Schematics of microglia depletion in cx3cr1CreER±:: R26iDTR± mice. Vehicle: corn oil; TAM: tamoxifen dissolved in corn oil. b Representative brain sections with IbaI+ cells (green). Scale bars, 350 µm. c Quantification of the microglia density in b (n = 2 mice/group). d Tissue ELISA of LCN2 at 6 h after surgery (n = 5 mice/group). e Tissue ELISA of IL-6 at 6 h after surgery (n = 5 mice/group). f Serum ELISA of IL-6 at 6 h or 24 h after surgery (n = 5 mice/group). g Percent of time spent in freezing behavior (n = 12 mice/group). Data are represented as mean ± SEM; NS, no significance; ** P < 0.01; **** P < 0.0001