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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Role of SPAK–NKCC1 signaling cascade in the choroid plexus blood–CSF barrier damage after stroke

Fig. 1

Stroke-induced phosphorylated activation of SPAK–NKCC1 complex in the choroid plexus. a Experimental protocol. Changes of SPAK–NKCC1 protein complex in the lateral ventricle choroid plexus (LVCP) from Sham or tMCAO mice were assessed by immunostaining, as illustrated in representative images of contralateral (CL) and ipsilateral (IL) LVCP stained for the NKCC1 protein. b Representative confocal fluorescence images of SPAK and NKCC1 expression. Arrowheads: apical membrane (region of interest) expression of SPAK or NKCC1 protein. c Quantification summary. Data are mean ± SD (n = 8). ***p < 0.001, ****p < 0.0001. One-way ANOVA. d Representative confocal fluorescence images of the phosphorylated species (active) of pSPAK or pNKCC1 protein. Arrowheads: low level of pSPAK and pNKCC1 in Sham groups. Arrows: elevated pSPAK or pNKCC1 expression in the post-stroke ChP. e Quantification summary. Data are mean ± SD (n = 8). *p < 0.05, **p < 0.01, ****p < 0.0001. One-way ANOVA

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