Fig. 3
From: Role of SPAK–NKCC1 signaling cascade in the choroid plexus blood–CSF barrier damage after stroke
Pharmacological inhibition of SPAK–NKCC1 signaling complex increased choroid plexus tight junction integrity after ischemic stroke. a Representative confocal images of tight junction proteins (Claudin-5, ZO-1, and Claudin-1) as well as the epithelial marker cytokeratin in the IL LVCP in Sham, stroke Veh-control, ZT-1a- or BMT-treated mice at 24 h Rp. Arrowheads: expression of Claudin-5, ZO-1 and Claudin-1. Arrows: Low level expression of Claudin-5 or Claudin-1. b–d Summary data of Claudin-5, ZO-1 and Claudin-1 fluorescence intensity. Data are mean ± SD (n = 6, 4 male, 2 female). *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA. e Western blot analysis of ZO-1, Claudin-5 and Claudin-1 TJ protein expression in the IL LVCP of Sham, stroke Veh-control, ZT-1a- and BMT-treated mice at 24 h Rp. ChP tissue lysates were subjected to immunoblotting with the indicated antibodies. f Immunoblot quantitation. Data are means ± SD (n = 4, 2 male, 2 female). *p < 0.05, **p < 0.01. One-way ANOVA