Fig. 5

Pharmacological inhibition of SPAK–NKCC1 signaling reduced infiltration of immune cell in the ChP. a Representative flow cytometric plots of CD11b⁺CD45^lo or CD11b⁺CD45^hi myeloid cells from the isolated IL ChP at 3 days post-surgery with quantification of total number of CD11b⁺CD45^lo or CD11b⁺CD45^hi myeloid cells in the ChP. b and c Percentage of CD11b⁺CD45⁺Ly6G⁻Ly6C^hi and CD206⁺ and Ym-1⁺ cells gated within CD11b⁺CD45⁺ cells. d Total number of CD11b⁺CD45⁺Ly6G⁺ neutrophils in the ChP. e Representative flow cytometric plots and the total number of CD3⁺ T cells in the ChP. Data are mean ± SD (n = 6–7). *p < 0.05, **p < 0.01. One-way ANOVA. f Representative images of CD8⁺ T cells (arrows) and Iba1⁺ microglia cells (arrowheads) in the IL LVCP in Sham, stroke Veh-control, ZT-1a- or BMT-treated stroke mice at 24 h Rp. g Quantification of CD8⁺ T cells and Iba1⁺ microglia cells per one coronal section in the IL hemispheres. Data are mean ± SD (n = 6, 4 male, 2 female). **p < 0.01, ***p < 0.001. One-way ANOVA