Fig. 1

IL-13 secreting Mφs show upregulation of CCR2 and CCR5 gene expression but not C5aR. a–d Mφs were isolated from C57BL/6J mice. a IL-13 secretion by IL-13 Mφs was significantly higher than M0, M1, or M2 Mφs as determined by an ELISA. n = 9–13. b–d qPCR analyses showed that the gene expression of C5aR (b) was not changed, whereas IL-13 Mφs showed significant upregulation of CCR2 (c) and CCR5 (d) expression compared to both M0 and M2 Mφs. Data were normalized to M0 Mφs and represent mean ± SEM. n = 4–7. Kruskal–Wallis test with Dunn’s correction.*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001