Fig. 4
3-week-old and 8-week-old mice exhibit similar neuroinflammatory responses to intracranial ZIKV infection. A qRT-PCR analysis of ZIKV RNA in the brains of mice at indicated day post-infection (dpi) following intracranial infection with ZIKV-MR766 (104 PFU). Values are reported in plaque forming unit-equivalents (PFUe) per hemisphere of brain tissue. n = 5–7 mice/group. B–K Flow cytometric analysis of infiltrating immune cells in brains of mice at 4dpi following intracranial ZIKV-MR766 infection. B Representative flow cytometry plots showing percentages of infiltrating (CD45hi) vs. resident (CD45lo) immune cells. C–G) Quantification of absolute numbers of myeloid cell populations expressing indicated markers. H) Representative flow cytometry plots showing CD45hi CD3+ cells stratified by expression of T cell markers CD4 and CD8. I–K Quantification of absolute numbers of CD45hi CD3+ cell populations expressing indicated markers. n = 3–4 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001. Bars represent group means