Fig. 5

Adoptive transfer of 2D2 CD4⁺Vα3.2⁺c-Met⁺ Th1 cells induces more severe EAE through Itgα4 overexpression. A EAE was induced by i.p. transfer of 3 × 10⁶ 2D2 Vα3.2⁺CD4^Crec-Met⁻ or c-Met⁺ or 2D2 Vα3.2⁺CD4^CreItgα4^fl/fl c-Met⁻ or c-Met⁺ Th1 followed by i.v. injection of PTX (67 ng/mouse) at d0 and d2. Data are representative of 2 independent experiments and mean clinical score were shown for n = 10 mice/group by two-way ANOVA with Bonferroni post Hoc test, *p < 0.05, **p < 0.01, ****p < 0.0001. B Representative flow cytometric histograms and quantification of c-Met-expressing CD4⁺Vα3.2⁺ infiltrating T cells at day 21 post-adoptive transfer. Data are representative of 2 independent experiments and mean values ± SEM were shown for n = 3 mice/group; ****p ≤ 0.0001 by unpaired two‐tailed Student's t‐test. C Quantification of CNS CD4⁺Vα3.2⁺IFNγ⁺ infiltrating T cells by flow cytometry at day 18 post-Th1 transfer. Data are representative of 2 independent experiments and mean values ± SEM for n = 3 mice/group are shown; *p ≤ 0.05, **p ≤ 0.01 by two-way ANOVA followed by Tukey’s post hoc test. D Representative flow cytometric histograms and quantification of Itgα4-expressing CD4⁺Vα3.2⁺ infiltrating T cells at day 18 post-adoptive transfer. Data are representative of 2 independent experiments and mean values ± SEM were shown for n = 3 mice/group; *p ≤ 0.05 by unpaired two‐tailed Student's t‐test