Fig. 1From: An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injuryPurification of Schwann cells after sciatic nerve crush. A Schematic of local response to sciatic nerve crush. Immediately after crush (day 0), debris and inflammatory cytokines are abundant. By 3Â days, macrophages proliferate at the site of injury and Schwann cells adopt a reparative phenotype. 7Â days after crush, most debris has been engulfed and Schwann cells align. B Purification of Schwann cells by immunopanning. Macrophages and perineural fibroblasts were depleted with anti-CD45 and anti-Thy1 antibodies, respectively. Schwann cells were positively selected by anti-O4 hybridoma. C Purified Schwann cells in culture after immunopanning. D Experimental timeline. Sciatic nerve crush occurred at day 0. At days 3, 7, and 7 post-crush, both whole nerve and purified Schwann cells were collected for downstream RNA-sequencingBack to article page