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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury

Fig. 1

Purification of Schwann cells after sciatic nerve crush. A Schematic of local response to sciatic nerve crush. Immediately after crush (day 0), debris and inflammatory cytokines are abundant. By 3 days, macrophages proliferate at the site of injury and Schwann cells adopt a reparative phenotype. 7 days after crush, most debris has been engulfed and Schwann cells align. B Purification of Schwann cells by immunopanning. Macrophages and perineural fibroblasts were depleted with anti-CD45 and anti-Thy1 antibodies, respectively. Schwann cells were positively selected by anti-O4 hybridoma. C Purified Schwann cells in culture after immunopanning. D Experimental timeline. Sciatic nerve crush occurred at day 0. At days 3, 7, and 7 post-crush, both whole nerve and purified Schwann cells were collected for downstream RNA-sequencing

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