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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury

Fig. 4

In situ hybridization spatial validation. A–D Four genes (GFRA1, MDK, GPC1, and SPARCL1) were selected for validation based on their clear response to injury in Schwann cells and lack of change in myeloid populations after injury. Sciatic nerves were counterstained with S100 (red) to confirm Schwann cell localization of expression changes. All four genes exhibit expected temporal expression patterns within the nerve (GFRA1, MDK, GPC1 increasing and SPARCL1 decreasing). Right, 63× zoom. Arrowheads highlight examples of S100 colocalization with the in situ probe for each candidate gene. Scales = 50 μm (left images), and 20 μm (right images)

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