Fig. 5

Inflammatory genes show distinct epigenetic regulation in ‘acute’, ‘tolerant’, ‘primed’ and ‘trained’ microglia. a Venn diagram of the enriched genes in acute (PL versus PP, light purple, Additional file 5), tolerized (clusters 2 and 4, dark purple, Additional file 7), primed (KO-PBS versus WT-PBS, mint, Additional file 6) and trained (KO-LPS versus WT-LPS, dark mint, Additional file 6) microglial response. b Dotplot depicting the GO terms associated with unique and overlapping gene sets of acute, tolerant, primed and trained microglia. The size of the dot represents the gene count per GO term and the color indicates the adjusted P-value. c Heat map depicting row z-scores of weighted mean differences (adjusted P value < 0.001) of ATAC peaks at loci of specific TF motifs in the indicated comparisons identified with diffTF (based on Figs. 3B, D, 4C, D; Additional files 10, 12). Only accessible TF motifs of activating, repressing and undetermined TF are displayed (not-expressed TFs were filtered out). White space indicates non-significant weighted mean differences of ATAC peaks at the given locus and/or not-expressed TFs in the indicated comparison.