Fig. 4

Female AD/MxD mice have greater Aβ, but not tau, pathology. A Representative image of Aβ plaques. B The number of Aβ plaques/brain section (averaged across 8, 40-μM-thick brain sections between -1.46 and -3.52 mm from bregma). C Hippocampal insoluble Aβ-40, measured by ELISA. D Hippocampal insoluble Aβ-42, measured by ELISA. E Representative Western blot images of tau protein in right (ischemic for MxD) hippocampal isolates. Measurements for F total tau, G pTau S199, and H pTauPHF1 were normalized to GAPDH. Data are presented as mean + SEM, #p < 0.05 effect of sex, ##p < 0.01 effect of sex, 3-way ANOVA, (n = 4–5/group)