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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Z-DNA binding protein 1 mediates necroptotic and apoptotic cell death pathways in murine astrocytes following herpes simplex virus-1 infection

Fig. 2

ZBP1 mediates HSV-1-induced cell death in primary murine astrocytes. ZBP1+/+ and ZBP1−/− murine astrocytes were infected with HSV-1(MacIntyre), HSV-1(F)-ICP6-RHIM Mut, or its parental ICP6-expressing parental strain (HSV-1(F)). One hour following infection, cells were treated with DMSO vehicle control, the RIPK1 inhibitor GSK963 (1 μM), the RIPK3 inhibitor GSK872 (5 μM), and/or the pan caspase inhibitor zVAD-FMK (20 μM). Cell viability was measured every two hours with a RealTime-Glo™ MT assay beginning at two hours following infection and data are reported as the percentage of dead cells at 24 h relative to non-infected controls (cell death) and as the rate of cell death. Data are shown as the mean of 3–6 independent experiments ± SEM. Asterisks indicate a significant difference from similarly treated ZBP1+/+ cells, while dagger symbols indicate significant difference from similarly challenged cells treated with DMSO vehicle only

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