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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Z-DNA binding protein 1 mediates necroptotic and apoptotic cell death pathways in murine astrocytes following herpes simplex virus-1 infection

Fig. 5

ZBP1-mediated cell death does not exclusively occur in HSV-1-infected astrocytes via necroptosis. ZBP1+/+ or ZBP1−/− murine astrocytes were infected with HSV-1(MacIntyre), HSV-1(F)-ICP6-RHIM Mut, or its parental ICP6-expressing parental strain (HSV-1(F)). One hour following infection, cells were treated with either the RIPK3 inhibitor GSK872 (5 μM) (A, B) or the pan caspase inhibitor zVAD-FMK (20 μM) (C, D). A, C Cell viability was measured every two hours with a RealTime-Glo™ MT assay beginning at two hours following infection. B, D At 24 h, total cell lysates were collected and analyzed for the presence of phosphorylated MLKL (P-MLKL) or the housekeeping gene product β-actin (Actin) by immunoblot analysis. Relative P-MLKL expression was determined by densitometric analysis and normalized to β-actin expression levels. Data are shown as the mean of three independent experiments ± SEM. Asterisks indicate a significant difference in final cell death from similarly treated ZBP1+/+ cells

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