Fig. 6

ZBP1 mediates both apoptotic and necroptotic cell death pathways in HSV-1 challenged primary murine astrocytes. ZBP1+/+ or ZBP1−/− derived astrocytes were infected with HSV-1(MacIntyre), HSV-1(F)-ICP6-RHIM Mut, or its parental ICP6-expressing parental strain (HSV-1(F)). One hour following infection, cells were treated with either the RIPK1 inhibitor GSK963 (1 μM) plus the pan caspase inhibitor zVAD-FMK (20 μM) (A) or the RIPK3 inhibitor GSK872 (5 μM) plus zVAD-FMK (20 μM) (B). Cell viability was measured every two hours with a RealTime-Glo™ MT assay beginning at two hours following infection. Data are shown as the mean of 4–6 independent experiments ± SEM. Asterisks indicate a significant difference in final cell death from similarly treated ZBP1+/+ cells