Fig. 2

Single-cell RNA sequencing on mouse hypothalamus after injected with ACP cystic fluid or PBS. A Schematic diagram of the establishment of the ACP cystic fluid-hypothalamus injury animal model and single-cell RNA sequencing of mouse hypothalamus and childhood ACP tissues. B UMAP image of single-cell RNA sequencing of the mouse hypothalamus. According to known specific marker genes, neurons (clusters 0, 1, 3, 5, 8, and 9), oligodendrocytes (clusters 2 and 10), oligodendrocyte premise cells (cluster 6), astrocytes (clusters 4 and 13), tanycytes (cluster 12), microglia (clusters 7, 11, and 15), pia mater cells (cluster 14), and dural cells (cluster 16) were identified. C Marker genes specifically expressed in each cell population and the corresponding cell population. Neurons (snap25+/syt1+), microglia (csf1r+/cx3cr1+), astrocytes (agt+), oligodendrocytes (cldn11+), oligodendrocyte precursor cells (pdgfra+/olig1+), tanycytes (rax+/crym+), dural cells (mgp+), and pia mater cells (lum+). D A portion of the feature plot analysis results, which allows intuitive visualization of the expression of specific marker genes in specific clusters. E GO enrichment map of some clusters. Further clarification of the identity of astrocytes (clusters 4 and 13), tanycytes (cluster 12), pia mater cells (cluster 14), and dural cells (cluster 16). F The ARC-specific marker gene TBX3 is highly expressed in cluster 3. G In cluster 3, CF (cystic fluid) vs. sham, the expression of the Pcsk1n and Sst genes in the cystic fluid group was significantly upregulated, and the expression of the Fgfr2 and Rnpc3 genes was significantly downregulated. H The number of genes related to feeding behavior, positive regulation of neuronal apoptosis, and APP metabolic regulation in cluster 3 of the cystic fluid group was significantly higher than that of cluster 3 of the sham operation group