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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3

Fig. 3

IL-13Rα1 on microglia/macrophages may play a key role in stroke brain. A qPCR measurement of the mRNA expression levels of pro-inflammatory markers Aa, b and anti-inflammatory markers Acd in the brain 3 days after tMCAO or sham operation. Data are shown as fold change of sham-vehicle controls. n = 4–5/group. B The expression of IL-13 receptors in the brain assessed by flow cytometry 3 days after tMCAO. Ba Gating strategy for the IL-13 receptors in the brain. Bb The fluorescence expression of IL-13Rα1 and IL-13Rα2 (Bc-f) observed in astrocytes/other cells (A), oligodendrocytes (OL), neurons (N), microglia (MG), and macrophages (MΦ/aMG). Bc, d Quantification of IL-13Rα1+ cells in the brain by flow cytometry. Be, f Quantification of IL-13Rα2+ cells in the brain by flow cytometry. n = 5/group. C Representative images of IL-13Rα1 (green) and Iba1 (red) immunostaining in the striatum 3d after tMCAO. Scale bar: 50 µm. D In vitro experiments. Da Quantification of primary neuron survival after 1 h OGD/R challenge. n = 4–6/group. Db Quantification of in vitro primary neuron survival 1 h after OGD/R in a transwell system (co-cultured with Veh/IL-13 treated primary microglia). n = 6–7/group. All data are presented as the mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Unpaired Student’s t-test (Bc), Mann–Whitney U test (Be), two-way ANOVA followed by Bonferroni’s post hoc (Bd, f), and one-way ANOVA followed by Bonferroni’s post hoc (A, D)

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