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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3

Fig. 8

IL-13 promotes white matter integrity after stroke at least partially by inhibiting STAT3 activation. A Double-immunostaining for MBP and SMI32 35d after tMCAO. Aa, b Representative images of MBP (green) and SMI32 (red) immunostaining in the peri-infarct EC and STR. Scale bar: 50 µm. Ac The Pearson correlation between the behavior tests and histological analysis of myelin in the STR or EC. n = 4–5/group. Ad, e Quantification of the MBP fluorescence intensity and the ratio of SMI32 to MBP immunofluorescence intensity in ipsilateral EC and STR. n = 4–5/group. B Double-immunostaining for Caspr and Nav1.6 in the peri-infarct EC 35d after tMCAO. Ba Representative images of Caspr (red) and Nav1.6 (green) immunostaining. Scale bar: 5 µm. Yellow arrow: Typical NORs. White arrow: damaged NORs. Bb, c Quantification of the number of nodes of Ranvier (NOR) and the paranode length. Bd Violin plot of the paranode length and Be frequency histogram of the paranode length as a function of NORs. n = 252 NORs from six animals for sham + Vehicle; n = 251 NORs from six animals for sham + IL-13; n = 240 NORs from six animals for tMCAO + Vehicle; n = 246 NORs from six animals for tMCAO + IL-13; n = 241 NORs from six animals for tMCAO + Stattic; n = 239 NORs from six animals for tMCAO + stattic + IL-13. Bf Quantification of the length of paranode gap. n = 6/group. All data are presented as the mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. One-way ANOVA followed by Bonferroni’s post hoc or Kruskal–Wallis test followed by Dunn’s post hoc (Ad, e). One-way ANOVA followed by Bonferroni’s post hoc (Bb-d), Kruskal–Wallis test followed by Dunn’s post hoc (Bf), and Pearson correlation (Ac)

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