Fig. 9

IL-13 promotes oligodendrogenesis after stroke at least partially by inhibiting STAT3 activation. A Double-immunostaining for BrdU and APC 35d after tMCAO. Aa illustrates where images in b were taken from. Ab Representative images of BrdU (green) and APC (red) immunostaining in the peri-infarct STR and EC. Scale bar: 50 µm. White squares illustrate the location of 3D-rendered images magnified. Ac–e Quantification of the number of BrdU⁺ cells and BrdU⁺APC⁺ cells in the peri-infarct STR and EC. n = 4/group. B OL/OPC survival after OGD/R was quantified by live/dead staining. Ba Representative images of live (green) and dead (red) staining of cultured oligodendrocytes or OPCs. Scale bar: 50 μm. Bb, c Percentages of dead OLs and OPCs were quantified. Data are from 3 independent experiments. C NG2 and MBP immunostaining was performed to evaluate OPC differentiation. Ca Percentages of MBP⁺ OLs were quantified. Data are from 3 independent experiments. Cb Representative images of NG2 (green), MBP (red) and DAPI (blue) staining of cultured oligodendrocytes or OPCs. Scale bar: 25 μm. All data are presented as the mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. One-way ANOVA followed by Bonferroni’s post hoc, or Kruskal–Wallis test followed by Dunn’s post hoc