Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway

Fig. 2

Effects of IF on microglia activation and inflammatory factors release after ICH. A Examples of Iba-1 immunohistochemistry in the ipsilateral basal ganglia at different timepoints after ICH, Scale bar = 50 μm. B The number of Iba-1+ cells was quantified as shown in the bar graphs (n = 6). Secretion of IL-1β C and TNF-α D in blood after ICH was measured by ELISA (n = 6). mRNA levels of IL-1β E and TNF-α F in microglia isolated from ipsilateral cerebra were measured by qRT-PCR. Double fluorescent staining of CD16 (G, Red) or Arg1 (H, Red) with Iba-1 (Green) in the ipsilateral basal ganglia respectively (Scale bar = 20 μm) and counting of CD16+Iba-1+ or Arg1+Iba-1+ cell numbers (n = 6). Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s test for multiple comparisons (B–F), Student t test between two groups (I, J). Values are mean ± SD, *P < 0.05 compared with ICH group

Back to article page