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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway

Fig. 4

Role of microglial Sirt3 in IF-induced neuroprotective effects after ICH. Cylinder test A and corner test B tests were performed pre-ICH (n = 41) and at day 1 (n = 38), 3 (n = 27), 7 (n = 18) and 28 (n = 9) after ICH in Sirt3f/f or Sirt3f/fCx3cr1-Cre mice with IF treatment. C Representative HE staining images of the largest clot at day 3 and 7 days after ICH with IF treatment, Scale bar = 1 mm. Quantification of hematoma size D and brain volume E in Sirt3f/f or Sirt3f/fCx3cr1-Cre mice after ICH with IF (n = 6). F Examples of DARPP-1 immunohistochemistry in the basal ganglia at 28 day after ICH with IF treatment, Scale bar = 1 mm. G Quantification of ipsilateral shrinkage of the DARPP-32 positive area in the mice (n = 6). H TUNEL staining of ipsilateral mice brain at day 1, 3 and 7 after ICH with IF treatment and I the assessment of the number of TUNEL positive cells (n = 6), Scale bar = 50 μm. Statistical analysis was performed using two-way ANOVA analysis plus post hoc Bonferroni’s test for behavioral data (A, B), one-way ANOVA followed by post hoc Tukey’s test for multiple comparisons (D, E), Student t test between two groups (G, I). Values are mean ± SD, #P < 0.05 compared with Sirt3f/f group

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