Fig. 7
Effects of Nrf2/HO-1 signaling pathways in IF-regulated microglia activation after ICH in Sirt3 cKO mice. A Western blot analysis of HO-1 and Nrf2 protein levels in microglia of ipsilateral basal ganglia of Sirt3f/f or Sirt3f/f cKO mice after ICH treated with or without and quantification of relative expression of HO-1 B and Nrf2 (C). D Examples of HO-1 immunohistochemistry in the ipsilateral basal ganglia of Sirt3f/f or Sirt3f/f cKO mice after ICH with or without IF treatment, Scale bar = 50 μm. E HO-1+ cells were quantitated in the bar graph (n = 6). F Western blot analysis of HO-1 and Nrf2 protein levels in microglia of ipsilateral basal ganglia and quantification of relative expression of HO-1 G and Nrf2 (H). Quantification of IL-1β I and TNF-α J in blood after ICH treated with IF plus vehicle control or DMF. Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s test for multiple comparisons (B, C, E), Student t test between two groups (I–J). Values are mean ± SD, #P < 0.05 compared with Sirt3f/f group, +P < 0.05 compared with ICH + IF + Vehicle group