Fig. 3

Differential expression analysis and gene ontology overrepresentation tests. A Principal component analysis. Each dot represents a sample. The values in the axis labels represent proportion of overall variance as described by each principal component, with PC1 describing the predominant amount of variance (39.1%). B Scatter plot of differential gene regulation. Each dot represents a gene expressed in at least WT, GFAP-IL6 or GFAP-IL6 × Sbno2^−/−. The x axis represents the fold-change (log₂) of gene expression in GFAP-IL6 when compared with in WT, the y axis represents the fold-change (log₂) of gene expression in GFAP-IL6 × Sbno2^−/− when compared with in WT. Blue dots (DE gene) are genes differentially expressed (a significant fold change (log₂) of ≥ 1 or ≤ − 1) between GFAP-IL6 and GFAP-IL6 × Sbno2^−/− mice, grey dots (non-DE gene) are genes which are not. Genes which were equally expressed in both GFAP-IL6 and GFAP-IL6 × Sbno2^−/− when compared with WT fall along the diagonal. C Top 20 of 275 biological process gene ontology terms enriched amongst genes upregulated in GFAP-IL6 × Sbno2^−/− mice compared with GFAP-IL6 mice. The top x axis (bars) represents fold enrichment, the bottom x axis (dots) represents the FDR-adjusted p value (log₁₀), dotted line represents FDR-adjusted p value of 0.05