Fig. 5

Immunoblot and RPA using hyper-IL-6-treated primary cultured astrocytes. A Decreased signal pathway phosphorylation in primary astrocytes treated for 6 h with hyper-IL-6. Immunoblot was performed on protein lysates from WT and Sbno2−/− primary cultured astrocytes untreated (UT) or treated with hyper-IL-6 (hyIL-6) for 6 h to determine the levels of given proteins (n = 4 for each genotype). Values from densitometric analysis were normalized to the corresponding GAPDH loading control. B RPA was performed on total RNA from WT and Sbno2^−/− primary cultured astrocytes to determine the level of RNA transcript for given genes (n = 3 for each genotype). * p < 0.05 between genotypes at each time point. *, p < 0.05 compared with untreated of same genotype; ^, p < 0.05 compared with corresponding WT