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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Strawberry notch homolog 2 regulates the response to interleukin-6 in the central nervous system

Fig. 6

RPA using primary cultured astrocytes treated with hyper-IL-6-treated and actinomycin D (ActD). WT and Sbno2−/− primary cultured astrocytes were treated with hyper-IL-6 for 2 h then treated with ActD (arrow) which inhibited transcription to determine transcript stability. RPA was performed on total RNA to determine the level of RNA transcript for given genes (n = 3). DMSO was used as a vehicle. Linear regression was performed using ActD values between 2 and 4 h and comparing genotypes using ANCOVA analysis [70, 71]. *, p < 0.05 between genotypes

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