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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Melatonin, an endogenous hormone, modulates Th17 cells via the reactive-oxygen species/TXNIP/HIF-1α axis to alleviate autoimmune uveitis

Fig. 6

Melatonin suppressed Th17 cells from EAU mice via the oxidative stress/TXNIP/HIF-1α axis. A Reactive-oxygen species (ROS) MFI were analyzed by flow cytometry. B Effects of different concentrations of melatonin on ROS MFI during early activation of naive CD4 + T cells. C Western blot analyses TXNIP and HIF-1α in the CD4 + T cells from DLNs of EAU mice. D DLN cells from EAU mice were stimulated for 72 h with 10 μg/mL IRBP1-20 under Th17-polarizing conditions in the absence (vehicle) or presence of melatonin (2, 20, or 200 ng/mL). CD4 + T cells were assessed for ROS MFI by flow cytometry. E DLN cells from EAU mice were stimulated for 72 h with 10 μg/mL IRBP1-20 under Th17-polarizing conditions in the absence (vehicle) or presence of melatonin (2, 20, 200 ng/mL). Th17-polarized DLN cells was assessed for TXNIP and HIF-1α expression via western blot, respectively. The representative data from three independent experiments. Significance was determined by unpaired t test (A), or one-way ANOVA (BE).*P < 0.05, **P < 0.01, ***P < 0.001

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