Skip to main content
Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: A breakdown in microglial metabolic reprogramming causes internalization dysfunction of α-synuclein in a mouse model of Parkinson’s disease

Fig. 4

Microglia-specific TRPV1 deficiency accelerates PFF-induced PD-like pathology in TRPV1flox/flox; Cx3cr1Cre mice. a Representative TEM images of α-synuclein monomers, pre-sonicated PFFs and post-sonicated PFFs. Scale bar, 200 nm. b Schematic diagram showing PFF experimental design. IF, immunofluorescence; IHC, immunohistochemistry. cg Behavioral assessment of TRPV1flox/flox and TRPV1flox/flox; Cx3cr1Cre mice following a single unilateral inoculation of PBS or PFF into the striatum. PFF-injected TRPV1flox/flox (n = 11) and TRPV1flox/flox; Cx3cr1Cre mice (n = 9), as well as age-matched PBS-injected TRPV1flox/flox (n = 10) and TRPV1flox/flox; Cx3cr1Cre animals (n = 7) are also shown. cd Rotarod test, and eg open field test. hk Representative immunohistochemistry images h and quantification of p-α-syn (Ser129) aggregates in SNpc (i), hippocampus j and cortex k of mice killed 6 months after striatal PFF injection (n = 3 animals per group). Scale bar, 50 μm. One-way ANOVA was used for statistical analysis followed by Tukey’s multiple comparisons test in dk. Two-way ANOVA was used for statistical analysis followed by Tukey’s multiple comparisons test in c. Error bars represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Back to article page