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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: A breakdown in microglial metabolic reprogramming causes internalization dysfunction of α-synuclein in a mouse model of Parkinson’s disease

Fig. 5

Microglia-specific TRPV1 deficiency accelerates loss of dopaminergic neurons in the SNpc of PFF-injected TRPV1flox/flox; Cx3cr1Cre mice. a Representative images of TH-immunostaining and b quantitative analysis of dopaminergic neurons in the SNpc region (n = 3, biological replicates). Scale bar, 200 μm or 50 μm, respectively. c Immunoblots of TH, p-α-syn (Ser129), α-syn, GFAP (GA5), Iba-1 and β-actin and d quantification in SNpc tissues of PBS- or PFF-injected TRPV1flox/flox mice or TRPV1flox/flox; Cx3cr1cre mice (n = 3, biological replicates). e Differentially expressed genes associated with transmission across chemical synapses (Slc22a2 and Nms) in the brains of TRPV1flox/flox or TRPV1flox/flox; Cx3cr1cre mice 6 months after intrastriatal injection with PFF (n = 3, biological replicates). fh Heat map representing relative expression levels of genes associated with anti-apoptosis (f), myelination g and synaptic transmission h (n = 3, biological replicates). i Module–trait relationships between gene modules implicated by WGCNA (n = 3, biological replicates). Data are presented as the correlation coefficient (p value). jm Bar graphs showing the results for GO terms and KEGG pathway enrichment analysis. The top 15 BPs in the purple j and top 20 BPs in the turquoise l modules are listed. Box plot graphs show the module eigengenes of the purple k and turquoise m modules. One-way ANOVA with Tukey’s multiple comparisons test was used for statistical analysis in b, d, k, and m. Student’s t-test was used for statistical analysis in e. Error bars represent mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001

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