Fig. 6

Microglia-specific TRPV1 deficiency accelerates α-syn PFF-induced metabolic defects and immune dysfunction in SNpc of PFF-injected TRPV1^flox/flox; Cx3cr1^Cre mice. a Two-way heat map cluster analysis, b volcano plot (log₂ (fold change) < − 0.585 or log₂ (fold change) > 0.585, −log₁₀ (p value) > 1.30), and c GO pathway analysis of differentially expressed genes from PFF-injected TRPV1^flox/flox; Cx3cr1^cre mice compared with PBS-injected TRPV1^flox/flox; Cx3cr1^cre mice (n = 3, biological replicates). d Two-way heatmap cluster analysis, e volcano plot (log₂ (fold change) < − 0.585 or log₂ (fold change) > 0.585, −log₁₀ (p value) > 1.30) and f GO pathway analysis of differentially expressed genes from PFF-injected TRPV1^flox/flox; Cx3cr1^cre mice compared with PFF-injected TRPV1^flox/flox mice (n = 3, biological replicates). g Bubble plot of KEGG pathway enriched by differentially expressed genes in brains of PFF-injected TRPV1^flox/flox; Cx3cr1^cre mice and TRPV1^flox/flox mice. h–k Western blot analysis of metabolic energy pathway (h, i), and mitophagy markers j, k in SNpc tissues (n = 3 mice, biological replicates). One-way ANOVA was used for statistical analysis followed by Tukey’s multiple comparisons test. Error bar represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001