From: Microglial amyloid beta clearance is driven by PIEZO1 channels
iPSC line | Sex | Health status | Age years | Method | Karyo-type | Refs | Assays |
---|---|---|---|---|---|---|---|
MAD1 clone 7 | M | Healthy | 67 | Sendai Virus 2.0 | 46 XY Normal | Additional file 1: Fig. S6 | Ca-imaging, LDH, MTT, Mitostress, Migration, pHrodo/Aβ phagocytosis |
MAD6 clone 1 | M | Healthy | 63 | Sendai Virus 2.0 | 46XY Normal | Fagerlund et al. (2021) [27] | Ca-imaging, LDH, MTT, Mitostress, LysoView, Migration, pHrodo/Aβ phagocytosis |
MAD8 clone 1 | M | Healthy | 64 | Sendai Virus 2.0 | 46 XY Normal | Additional file 1: Fig. S6 | Ca-imaging, Cytotox, LDH, MTT, Mitostress, pHrodo/Aβ phagocytosis |
Ctrl 8.2 | F | Healthy | Adult | Sendai Virus 1.0 | Â | Holmqvist et al. (2016) [105] | Cytotox |
MBE2968 clone 1 | F | Healthy | 65 | Episomal nucleofection | 46XX Normal | Konttinen et al. [26] | All assays, Ca-imaging, Cytotox, LDH, MTT, Mitostress, CBA, LysoView, pHrodo/Aβ phagocytosis |
MBE2960 polyclonal | M | Healthy | 78 | Episomal nucleofection | 46XY Normal | Munoz et al. (2020) [24] | Ca-imaging, LysoView, Mitostress |
TOB0644_F_B9_F3 | M | Healthy | 73 | Episomal nucleofection, CRISPR edited from APOE4/4 | 46XX Normal | Hernandez et al. (2021) [25] | Ca-imaging |
LL190 clone 1.5 | F | Healthy | 44 | Sendai Virus 2.0 | 46XX Normal | Oksanen et al. (2017) [23] | ICC |
Isogenic AD4 clone 1.6.12.10 | F | Corrected presymptomatic PSEN1DE9 (EOAD) | 47 | Sendai Virus 2.0, CRISPR corrected PSEN1DE9 | 46XX Normal | Oksanen et al. (2017) [23] | Ca-imaging, CBA |
Isogenic AD4 clone 1.5.6.1 | M | Corrected PSEN1DE9 (EOAD) | 48 | Sendai Virus 2.0, CRISPR corrected PSEN1DE9 | 46XY Normal | Oksanen et al. (2017) [23] | Ca-imaging, CBA |
BIONi010-C-2 | M | Healthy | 15–19 | Non-integrating episomal, CRISPR edited from APOE4/4 | 46 XY Normal | EBiSC | ICC, LysoView, pHrodo |