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Table 1 Data for human iPSC cell lines

From: Microglial amyloid beta clearance is driven by PIEZO1 channels

iPSC line

Sex

Health status

Age

years

Method

Karyo-type

Refs

Assays

MAD1 clone 7

M

Healthy

67

Sendai Virus 2.0

46 XY Normal

Additional file 1: Fig. S6

Ca-imaging, LDH, MTT, Mitostress, Migration, pHrodo/Aβ phagocytosis

MAD6 clone 1

M

Healthy

63

Sendai Virus 2.0

46XY Normal

Fagerlund et al. (2021) [27]

Ca-imaging, LDH, MTT, Mitostress, LysoView, Migration, pHrodo/Aβ phagocytosis

MAD8 clone 1

M

Healthy

64

Sendai Virus 2.0

46 XY Normal

Additional file 1: Fig. S6

Ca-imaging, Cytotox, LDH, MTT, Mitostress, pHrodo/Aβ phagocytosis

Ctrl 8.2

F

Healthy

Adult

Sendai Virus 1.0

 

Holmqvist et al. (2016) [105]

Cytotox

MBE2968 clone 1

F

Healthy

65

Episomal nucleofection

46XX Normal

Konttinen et al. [26]

All assays, Ca-imaging, Cytotox, LDH, MTT, Mitostress, CBA, LysoView, pHrodo/Aβ phagocytosis

MBE2960 polyclonal

M

Healthy

78

Episomal nucleofection

46XY Normal

Munoz et al. (2020) [24]

Ca-imaging, LysoView, Mitostress

TOB0644_F_B9_F3

M

Healthy

73

Episomal nucleofection, CRISPR edited from APOE4/4

46XX Normal

Hernandez et al. (2021) [25]

Ca-imaging

LL190 clone 1.5

F

Healthy

44

Sendai Virus 2.0

46XX Normal

Oksanen et al. (2017) [23]

ICC

Isogenic AD4 clone 1.6.12.10

F

Corrected presymptomatic PSEN1DE9 (EOAD)

47

Sendai Virus 2.0, CRISPR corrected PSEN1DE9

46XX Normal

Oksanen et al. (2017) [23]

Ca-imaging, CBA

Isogenic AD4 clone 1.5.6.1

M

Corrected PSEN1DE9 (EOAD)

48

Sendai Virus 2.0, CRISPR corrected PSEN1DE9

46XY Normal

Oksanen et al. (2017) [23]

Ca-imaging, CBA

BIONi010-C-2

M

Healthy

15–19

Non-integrating episomal, CRISPR edited from APOE4/4

46 XY Normal

EBiSC

ICC, LysoView, pHrodo

  1. All with APOE3/3 alleles and derived from fibroblasts of skin biopsies
  2. CBA cytokine bead array, EOAD early-onset Alzheimer’s disease, ICC immunocytochemistry, LDH lactate dehydrogenase, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, metabolic assay; PSEN1DE9 Presenilin 1 delta exon 9 deletion