Fig. 5

Effects of thrombin on the MIF production from astrocytes. a ELISA determination of MIF in the supernatants of primary astrocytes challenged by 0–200 nM thrombin for 24 h. b Western blot analysis of MIF protein levels in the astrocytes stimulated by 0–200 nM thrombin for 24 h. c Quantification data as shown in b. Quantities were normalized to endogenous β-actin. d MTT assay of hirudin effects on the cell viability of astrocytes. e Western blot analysis of MIF protein levels in the astrocytes treated by 0–10 U/ml hirudin in the presence of 100 nM thrombin. f Quantification data as shown in e. Quantities were normalized to endogenous β-actin. Experiments were performed in triplicates. Error bars represent the SEM (*P < 0.05)