Fig. 8

Effects of MAPKs and NFκB inhibition on the MIF production of astrocytes. a Western blot analysis of MIF following astrocyte treatment with 100 nM thrombin in the presence of 10 μM P38 (SB203580), 10 μM JNK (SP600125), or 10 μM ERK (PD98059) inhibitor for 24 h. b Quantification data as shown in a. Quantities were normalized to endogenous β-actin. c Western blot analysis of MIF following astrocyte treatment with 0–100 μM NFκB inhibitor PDTC in the presence of 100 nM thrombin for 24 h. d Quantification data as shown in c. Quantities were normalized to endogenous β-actin. Experiments were performed in triplicates. Error bars represent the SEM (*P < 0.05)