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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Activation of locus coeruleus-spinal cord noradrenergic neurons alleviates neuropathic pain in mice via reducing neuroinflammation from astrocytes and microglia in spinal dorsal horn

Fig. 1

Chemogenetic activation of LC neurons in vivo. A Experimental protocol for vector injection, immunofluorescence, and in vivo extracellular recordings in the LC. B Schematic representation of the Gq-DREADD protocol targeting LC neurons. C Expression of M3-mCherry in LC and its co-expression with LC noradrenergic (TH positive) neuron. Bar = 200 μm. D The discharge of the LC neurons recorded using a single electrode placed in the LC. E The firing frequency distribution diagram of neurons in the LC. F Changes of LC neuron firing before and 60 min after peritoneal injection of CNO. G Baseline firing frequency of LC neurons in Sham and CCI mice. H Intraperitoneal injection of CNO increased the firing frequency of LC neurons. I NE contents in SDH increased after LC:SC activation with Gq-DREADD. Data are expressed as mean + SEM, n = 6 for electrophysiological recordings and ELISA tests. **p < 0.01, ***p < 0.001. ns not statistically significant

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