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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Activation of locus coeruleus-spinal cord noradrenergic neurons alleviates neuropathic pain in mice via reducing neuroinflammation from astrocytes and microglia in spinal dorsal horn

Fig. 4

LC:SC activation for one week increases NE in SDH and relieves NP. A Experimental protocol for AAV injection, CCI surgery, and pain threshold test. i.t. intrathecal injection. i.p. intraperitoneal injection. B Schematic diagram of LC and spinal cord virus injection in mice. C The content of NE in the dorsal spinal cord that was measured by ELISA increased in CCI + M3 + CNO group compared with that of the CCI + M3 + Saline group. No significant differences were observed for the remaining groups. D On the 31st day, paw withdrawal threshold tested with von Frey filaments increased in CCI + M3 + CNO group and CCI + M3 + CNO + Saline group compared with that on the 21st day after vector injection. E On the 31st day, thermal withdrawal latency measured by a plantar thermal testing apparatus in CCI + M3 + CNO group and CCI + M3 + CNO + saline group compared with that on the 21st day after vector injection. Data are expressed as mean + SEM, n = 6 for NE content evaluation, n = 8 for mechanical withdrawal threshold tests and thermal withdrawal latency tests. **p < 0.01, ***p < 0.001, ns not statistically significant

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Journal of Neuroinflammation

ISSN: 1742-2094