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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke

Fig. 1

TFH cells infiltrate the ischemic brain. a Representative gating from ipsilateral brains harvested from SHAM mice, 4 h, 24 h, and 4 days post-tMCAO in WT mice. Following gating on single cells and live cells, we subsequently gated on cells double-positive for CD4 and ICOS-1. From the double-positive CD4+ ICOS-1+ cells, CXCR5 and IL-21 was gated based on isotype control for IL-21. bd Quantification of CD4+ T cells (b), percentage of CD4 T cells positive for ICOS-1 (c), and % of CD4+ ICOS-1+ T cells positive for both CXCR5 and IL-21 (d) in ipsilateral brains of SHAM mice and mice 4 h, 24 h, and 4 days post-tMCAO (n = 6). Data represent mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. n.s. not significant. Data are combined from three independent experiments. One-way ANOVA followed by Dunn’s post hoc test (bd)

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