Fig. 2

Diurnal changes in microglia and the inflammatory-related genes in SN of MPTP-induced Mice. A Representative images of IBA1 staining in the SN of control and MPTP mouse with IBA1 size quantification (B). Scale bar, 50 μm. Mice were killed at ZT6 and ZT18. C Iba1 staining in the control and MPTP group with Iba1 number quantification (D). Scale bar, 200 μm. E–H RT-PCR analysis for microglial inflammatory genes from SN of Control and MPTP mice. n = 3–4 for each group. Data were presented as mean ± SEM. (*p < 0.05, **p < 0.01, or ****p < 0.0001 by two-tailed t test)