Fig. 3

Activation of Rev-erbα inhibited microglial activation induced by MPP⁺ and αsyn PFF. The representative western blot bands (A) and the statistical graph (B–D) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, IL-18, IL-6, TNF-a, iNOS, Arg-1 and CD163 protein expressions. BV2 cells were pretreated with SR9009 (2 μM, 5 μM, 10 μM) for 1 h, then incubated with MPP⁺ for 24 h. The representative western blot bands (E) and the statistical graph (F–H) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, CD68 and αsyn protein expressions. BV2 cells were pretreated with SR9009 (2 μM, 5 μM, 10 μM) for 1 h, then incubated with αsyn pre-formed-fibril for 6 h. The p-NF-κB p65 level was normalized to the total of NF-κB p65, and the rest protein levels were normalized to β-actin. Data were presented as mean ± SEM (n = 3). (*p < 0.05, **p < 0.01, ***p < 0.001, **** or p < 0.0001 by One-way ANOVA test)