Table 1 Mouse primer sequences for quantitative real-time PCR (qPCR)
Gene | Forward (5ʹ-3ʹ sequence) | Reverse (3ʹ-5ʹ sequence) |
|---|---|---|
Rev-erbα | TTTTTCGCCGGAGCATCCAA | ATCTCGGCAAGCATCCGTTG |
IL-1β | AATGCCACCTTTTGACAGTGATG | AGCTTCTCCACAGCCACAAT |
IL-18 | TCAAAGTGCCAGTGAACCCC | GGTCACAGCCAGTCCTCTTAC |
IL-6 | ATCCAGTTGCCTTCTTGGGACTGA | TAAGCCTCCGACTTGTGAAGTGGT |
TNF-α | AGC AAA CCA CCA AGT GGA GGA | GCT GGC ACC ACT AGT TGG TTG T |
Nlrp3 | ATTACCCGCCCGAGAAAGG | TCGCAGCAAAGATCCACACAG |
Arg-1 | TGCTCACACTGACATCAACACTCC | TCTACGTCTCGCAAGCCAATGTAC |
iNOS | GGC AAA CCC AAG GTC TAG GTT | TCG CTC AAG TTC AGC TTG GT |
Actin | GCCTCACTGTCCACCTTCCA | AGCCATGCCAATGTTGTCTCTT |