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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: The primary macrophage chemokine, CCL2, is not necessary after a peripheral nerve injury for macrophage recruitment and activation or for conditioning lesion enhanced peripheral regeneration

Fig. 8

CCL2 is expressed primarily in macrophages in the distal sciatic nerve after injury. A–H Representative images of uninjured (A, C, E, G) and injured (B, D, F, H) distal nerves stained for the co-translated RFP to localize CCL2 expression at 2 (B, D) and 3 (F, H) DPI. Very little CCL2 is present at baseline (A, C, E, G). Many F4/80+ cells express CCL2 at both 2 (B, D; arrowheads) and 3 (F, H; arrowheads) DPI. Other cells that are likely Schwann cells and other stromal cells (arrows; B, D, F, H) also express CCL2 after injury. I–L Quantification of cells making CCL2 at 2 (I, J) and 3 (K, L) DPI. The majority of CCL2+ cells are F4/80+ macrophages at both 2 (I vs. J) and 3 (K vs. L) DPI, and CCL2+ macrophages increase over the course of the injury response (I vs. K). The lack of a decrease in non-macrophage CCL2+ cells in the DCKOs compared to controls (J, L) suggests Schwann cells make little contribution to CCL2 expression. Individual images for the F4/80 and RFP channels are also displayed below each merged image (A–H). Data are the mean ± SEM, n = 5 for the 2 DPI groups and n = 6 for the 3 DPI groups. # Indicates a significant (p < 0.05) difference between Sh and Ax within a genotype

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