Fig. 9

CCL2 is not required for macrophage recruitment either to the DRG or distal sciatic nerve 7 DPI. A–D Representative images showing macrophages marked by CD68 immunostaining in L4 DRGs ipsilateral (B, D) and contralateral (A, C) to a sciatic nerve transection. Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (B vs. D). E Macrophages were quantified by CD68 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 6, Ccl2 KO n = 8. F–I Representative images showing macrophages marked by Iba1 immunostaining in L4 DRGs ipsilateral (G, I) and contralateral (F, H) to a sciatic nerve transection. Again, Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (G vs. I). J Macrophages were quantified by Iba1 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 7, Ccl2 KO n = 8. K–N Representative images showing macrophages marked by CD68 immunostaining in distal sciatic nerves ipsilateral (L, N) and contralateral (K, M) to a sciatic nerve transection. Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (L vs. N). O Macrophages were quantified by CD68 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 9, Ccl2 KO n = 8. P–S Representative images showing macrophages marked by Iba1 immunostaining in distal sciatic nerves ipsilateral (Q, S) and contralateral (P, R) to a sciatic nerve transection. Again, Ccl2 KOs show an increase in macrophages after injury comparable to WT controls (Q vs. S). T Macrophages were quantified by Iba1 area and plotted as mean ± SEM. There are no differences between genotypes before or after injury. WT n = 8, Ccl2 KO n = 8. # Indicates a significant (p < 0.05) difference between Sh and Ax within a genotype. Scale bar is 50 μm