Fig. 4

Double-immunofluorescence staining of CRF-R1 (red fluorescence) A–F or CRF-R2 G–I (red fluorescence) with proopiomelanocortin (POMC) (green fluorescence) B, H and vasopressin (green fluorescence) E in the paraventricular nucleus (PVN) of the rat hypothalamus. A–C Double-immunofluorescence staining of coronal brain sections of the rat with hind paw inflammation showing that CRF-R1-immunoreactive neurons within PVN overlap with the opioid peptide precursor POMC (A–C). Some neurons express CRF-R1 or POMC only. D–F Show CRF-R1-immunoreactive neurons in the paraventricular nucleus (PVN) residing in close vicinity of vasopressin positive cells without any overlap of the two cell groups. G–I Show only few scattered CRF-R2-immunoreactive neurons without POMC overlap in the same region. Bar = 20 μm