Fig. 9

CRF-R1 and CRF-R2 mRNA expression in the hypothalamus region of the rat with 4 day FCA-induced hind paw inflammation. A RNA extraction from the rat hypothalamus, implementation of conventional PCR using specific primer pairs for CRF-R1 and CRF-R2, and subsequent visualization on a 2% agarose gel provided specific PCR products for the expression of CRF-R1 (280 bp) and CRF-R2 (230 bp) mRNA. B Shows the DNA melting profiles of the CRF-R1 (right) and CRF-R2 (left) specific primer pairs. C, D Quantification of CRF-R1 and CRF-R2 mRNA using Taqman® Real-Time PCR in the hypothalamus region of the rat brain. C The amplification profiles of the 18S- and CRF-R1- and CRF-R2-specific cDNA of rat hypothalamus. D The column graph representing % CRF-R1 mRNA expression relative to the expression of CRF-R2 mRNA. Note that CRF-R1 expression is more than threefold higher than that of CRF-R2 (experiments were done in duplicate from n = 5 rats, *P < 0.05, two-tailed independent Student t-test)