Fig. 4

Numbers of microglia in the white matter regions, overall, and stratified by severity of myelin loss. A Graph showing number of Iba1-positive cells in the intragyral white matter of the sagittal gyrus (SWM) and the first parasagittal gyrus (IGWM1) and second parasagittal gyrus (IGWM2) in sham control (n = 8), ischemia-normothermia (n = 8) and ischemia-hypothermia (n = 8) groups. *(p < 0.05) vs. sham control, ^{^}(p < 0.05) vs. ischemia-normothermia, repeated measures ANOVA and LSD post hoc test. B Graph showing number of Iba1-positive cells in the intact area in sham control (SC), intact area, lesion area and extensive diffuse area in ischemia-normothermia (IN) and intact and localized diffuse area in ischemia-hypothermia (IH). *(p < 0.05) vs. sham control intact, ^{^}(p < 0.05) vs. ischemia-normothermia intact, Kruskal–Wallis one-way ANOVA. Representative images of Iba1 in C the intact area in sham control, D intact area in ischemia-normothermia, E lesion area in ischemia-normothermia and F extensive diffuse area in ischemia-normothermia and G intact area in ischemia-hypothermia and H localized diffuse area in ischemia-hypothermia. Scale bar = 100 µm