Fig. 7

Microglial phenotype marker expression in the cortex and white matter. Graphs showing area fraction of CD86 and CD206 RNA puncta in the A cortex (PSC) and B intragyral white matter of the first parasagittal gyrus (IGWM) in sham control (n = 7), ischemia-normothermia (n = 4) and ischemia-hypothermia (n = 7) groups. C Graph showing the ratio of CD86 puncta to CD206 puncta in the PSC and IGWM. Representative images of Hoechst, Iba1 and CD86 for sham control D in the PSC and F in the IGWM and representative images of Hoechst, Iba1 and CD206 in E in the PSC and G in the IGWM showing low expression of both markers. *(p < 0.05) vs. sham control, ^{^}(p < 0.05) vs. ischemia-normothermia, one-way ANOVA and LSD post hoc test. Representative images of Hoechst, Iba1 and CD86 for ischemia-normothermia H in the PSC and J in the IGWM and representative images of Hoechst, Iba1 and CD206 in I in the PSC and K in the IGWM showing the upregulation of both markers compared with sham control. Representative images of Hoechst, Iba1 and CD86 for ischemia-hypothermia L in the PSC and N in the IGWM and representative images of Hoechst, Iba1 and CD206 in M in the PSC and O in the IGWM showing the upregulation of both markers compared with sham control but the suppression of CD86 in the PSC only compared with ischemia-normothermia. Arrows indicate the cell shown in each respective inset. Scale bar for O and inset = 50 µm