Fig. 9

Astrocyte area fraction in the cortex and white matter. A Graph showing GFAP area fraction for the cortex of the sagittal (SC), first parasagittal (PSC1) and second parasagittal cortex gyri (PSC2) in sham control (n = 8), ischemia-normothermia (n = 8) and ischemia-hypothermia (n = 8) groups. B Graph showing GFAP area fraction in the intragyral white matter of the sagittal (SWM), first parasagittal (IGWM1) and second parasagittal gyri (IGWM2). C Graph showing number of GFAP-positive cells in the cortex and intragyral white matter of the first parasagittal gyrus. *(p < 0.05) vs. sham control, ^{^}(p < 0.05) vs. ischemia-normothermia. Repeated measures ANOVA and LSD post hoc test for A and B, one-way ANOVA and LSD post hoc test for C. Representative GFAP whole section images for D sham control with higher GFAP expression in the white matter, E ischemia-normothermia with increased GFAP expression in the cortex and reduced in the white matter and F ischemia-hypothermia with normalization of GFAP expression distribution. Scale bar for F = 5 mm. Representative GFAP images for sham control in the G cortex and H white matter, ischemia-normothermia in the I cortex and J white matter and ischemia-hypothermia in the K cortex and L white matter. Scale bar for L = 200 µm. M Graph showing area fraction for NeuN, GFAP and Hoechst, one-way ANOVA and LSD post hoc test. N Graph showing no significant correlation between NeuN area fraction and GFAP area fraction, by linear regression. Representative images of Hoechst, GFAP and NeuN triple-labeling for O sham control, P ischemia-normothermia and Q ischemia-hypothermia. Scale bar for Q = 100 µm